An unbiased nuclear proteomics approach reveals novel nuclear protein components that participates in MAMP-triggered immunity

Fakih, Zainab, Ahmed, Md Bulbul, Letanneur, Claire et Germain, Hugo (2016). An unbiased nuclear proteomics approach reveals novel nuclear protein components that participates in MAMP-triggered immunity. Plant Signaling and Behavior, 11 (6). ISSN 1559-2316 1559-2324 DOI 10.1080/15592324.2016.1183087

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Résumé

ABSTRACT: (MAMP)-triggered immunity (MTI) is the first layer of molecular defense encountered by pathogens. Genetic screens have contributed to our knowledge of MTI, but are limited to phenotype-causing mutations. Here we attempt to identify novel factors involved in the early event leading to plant MTI by comparing the nuclear proteomes of two Arabidopsis genotypes treated with chitosan. Our approach revealed that following chitosan treatment, cerk1 plants had many nuclear accumulating proteins in common, but also some unique ones, when compared with Col-0 plants. Analysis of the identified proteins revealed a nuclear accumulation of DNA-modifying enzymes, RNA-binding proteins and ribosomal proteins. Our results demonstrate that nuclear proteomic is a valid, phenotype-independent approach to uncover factor involved in cellular processes. © 2016 The Author(s). Published with license by Taylor & Francis.

Type de document:	Article
Date de dépôt:	15 mars 2021 15:09
Dernière modification:	15 mars 2021 15:09
Version du document déposé:	Version officielle de l'éditeur
URI:	https://depot-e.uqtr.ca/id/eprint/9510

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