In situ architecture of the nuclear pore complex of the higher plant Arabidopsis thaliana

Sanchez Carrillo, I. B., Hoffmann, P. C., Obarska-Kosinska, A., Fourcassié, V., Beck, M. et Germain, H. (2025). In situ architecture of the nuclear pore complex of the higher plant Arabidopsis thaliana. Nature Plants . ISSN 2055-026X 2055-0278 DOI 10.1038/s41477-025-02138-y

Prévisualisation

PDF Disponible sous licence Creative Commons Attribution. Télécharger (9MB) | Prévisualisation

Résumé

Abtract

The nucleus is enclosed by the nuclear envelope, which contains nuclear pore complexes (NPCs). While NPCs have been well studied in vertebrates, yeast and algae, in situ structural data for higher plants is lacking. Here we show that individual nucleoporins of Arabidopsis thaliana and humans exhibit high structural similarity. We report an in situ NPC structure of higher plants, derived from A. thaliana root protoplasts using cryo-electron tomography, subtomogram averaging and homology-based integrative modelling. We present the AtNPC model based on predictions of A. thaliana nucleoporins (NUPs), supported by mass spectrometry. Here the AtNPC scaffold contains one Y-complex ring at the cytosolic and two at the nuclear ring. The AtNPC contains prominent NUP155 connector elements that are conserved in human NPCs but not in Chlamydomonas reinhardtii NPCs. Our model suggests that the ELYS homologue HOS1 plays an important role in the head-to-tail connection of Y-complexes in AtNPCs.

Type de document:	Article
Date de dépôt:	12 mars 2026 14:45
Dernière modification:	12 mars 2026 14:45
Version du document déposé:	Version officielle de l'éditeur
URI:	https://depot-e.uqtr.ca/id/eprint/12649

Actions (administrateurs uniquement)

Éditer la notice