A Gel-Free Budget-Friendly Approach to GFP-Tagged Viruses Quantification in Plant Samples

Grandhi, R., Plourde, M.-B., Balasubramani, A. et Germain, H. (2025). A Gel-Free Budget-Friendly Approach to GFP-Tagged Viruses Quantification in Plant Samples. Phyton-International Journal of Experimental Botany, 94 (5). pp. 1497-1504. ISSN 0031-9457 1851-5657 DOI 10.32604/phyton.2025.063974

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Résumé

Abstract
Viral diseases are an important threat to crop yield, as they are responsible for losses greater than US$30 billion annually. Thus, understanding the dynamics of virus propagation within plant cells is essential for devising effective control strategies. However, viruses are complex to propagate and quantify. Existing methodologies for viral quantification tend to be expensive and time-consuming. Here, we present a rapid cost-effective approach to quantify viral propagation using an engineered virus expressing a fluorescent reporter. Using a microplate reader, we measured viral protein levels and we validated our findings through comparison by western blot analysis of viral coat protein, the most common approach to quantify viral titer. Our proposed methodology provides a practical and accessible approach to studying virus-host interactions and could contribute to enhancing our understanding of plant virology.

Type de document:	Article
Mots-clés libres:	Microplate reader CP-PlAMV Viruses Plant viral quantification Green fluorescent protein Western blot quantification Nicotiana benthamiana Arabidopsis thaliana Pearson’s correlation
Date de dépôt:	28 juill. 2025 15:35
Dernière modification:	28 juill. 2025 15:35
Version du document déposé:	Version officielle de l'éditeur
URI:	https://depot-e.uqtr.ca/id/eprint/12167

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