Melampsora larici-populina homologous effectors Mlp72983 and Mlp52166 display cell-type specific accumulation in Arabidopsis and Populus

Gazal, S., dos Santos, K. C. G., Plourde, M. B., Levasseur, C., Séguin, A., Tanguay, P., Petre, B. et Germain, H. (2025). Melampsora larici-populina homologous effectors Mlp72983 and Mlp52166 display cell-type specific accumulation in Arabidopsis and Populus. Canadian Journal of Forest Research, 55 . ISSN 0045-5067 1208-6037 DOI 10.1139/cjfr-2024-0225

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Résumé

Abstract

Plants interact with microorganisms that can cause diseases and reduce crop productivity. The fungal pathogen Melampsora larici-populina (Mlp) causes the leaf rust disease of poplar trees by secreting proteins, termed effectors, into host tissues to promote pathogenesis. In this study, we functionally characterized two homologous Mlp candidate secreted effector proteins (CSEPs), Mlp72983 and Mlp52166. Confocal microscopy experiments revealed that Mlp72983 has cell type-specific differential localization. It accumulates in the guard cells’ chloroplast of the epidermis and in the nucleus of the spongy mesophyll of Arabidopsis thaliana and Populus alba x Populus tremula. Mlp52166 has a nucleocytosolic accumulation in the epidermal layer and has nuclear localization in the mesophyll layer of the two species. Transcriptomic experiments showed that Mlp72983 and Mlp52166 deregulate plant genes, when constitutively expressed in either Arabidopsis or poplar. The two CSEPs deregulate genes that encode histones, hydroxysteroid dehydrogenases, and multidrug and toxic compound extrusion proteins, with roles in DNA repair, methylation, and xenobiotic detoxification. Inoculation assays showed that CSEPs overexpression in poplar transgenics did not enhance susceptibility to rust infection. Despite being closely related in sequence identity, Mlp72983 and Mlp52166 have cell type-specific subcellular localization and deregulate mostly unique sets of plant genes.

Type de document:	Article
Mots-clés libres:	Pucciniales Epidermis layer Nucleus Subcellular localization Gene deregulation
Date de dépôt:	25 mars 2025 15:47
Dernière modification:	25 mars 2025 17:31
Version du document déposé:	Version officielle de l'éditeur
URI:	https://depot-e.uqtr.ca/id/eprint/11770

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