Specific alterations in riboproteomes composition of isonicotinic acid treated arabidopsis seedlings


Téléchargements par mois depuis la dernière année

Plus de statistiques...

Fakih, Z., Plourde, M. B., Nkouankou, C. E. T., Fourcassié, V., Bourassa, S., Droit, A. et Germain, H. (2023). Specific alterations in riboproteomes composition of isonicotinic acid treated arabidopsis seedlings. Plant Molecular Biology . ISSN 1573-5028 DOI 10.1007/s11103-022-01332-2

[thumbnail of GERMAIN_H_135_ED.pdf]
Télécharger (2MB) | Prévisualisation


Plants have developed strategies to deal with the great variety of challenges they are exposed to. Among them, common targets are the regulation of transcription and translation to finely modulate protein levels during both biotic and abiotic stresses. Increasing evidence suggests that ribosomes are highly adaptable modular supramolecular structures which remodel to adapt to stresses. Each Arabidopsis thaliana ribosome consists of approximately 81 distinct ribosomal proteins (RPs), each of which is encoded by two to seven genes. To investigate the identity of ribosomal proteins of the small subunit (RPS) and of the large subunit (RPL) as well as ribosomes-associated proteins, we analysed by LC/MS/MS immunopurified ribosomes from A. thaliana leaves treated with isonicotinic acid (INA), an inducer of plant innate immunity. We quantified a total of 2084 proteins. 165 ribosome-associated proteins showed increased abundance while 52 were less abundant. Of the 52 identified RPS (from a possibility of 104 encoding genes), 15 were deregulated. Similarly, from the 148 possible RPL, 80 were detected and 9 were deregulated. Our results revealed potential candidates involved in innate immunity that could be interesting targets for functional genomic studies.

Type de document: Article
Date de dépôt: 28 févr. 2023 16:53
Dernière modification: 28 févr. 2023 16:53
Version du document déposé: Version officielle de l'éditeur
URI: https://depot-e.uqtr.ca/id/eprint/10501

Actions (administrateurs uniquement)

Éditer la notice Éditer la notice