Semi-automated on-demand control of individual droplets with a sample application to a drug screening assay


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Hébert, M., Courtney, M. et Ren, C. L. (2019). Semi-automated on-demand control of individual droplets with a sample application to a drug screening assay. Lab on a Chip, 19 (8). pp. 1490-1501. ISSN 1473-0197 DOI 10.1039/c9lc00128j

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Automated control of individual droplets in microfluidic channels offers tremendous potential for applications requiring high accuracy and minimal user involvement. The feasibility of active droplet control has been previously demonstrated with pressure-driven flow control and visual feedback, but the manual operation required to perform droplet manipulations limited the accuracy, repeatability, and throughput. The present study improves upon the aforementioned challenges with a higher-level algorithm capturing the dynamics of droplet motion for a semi-automated control system. With a simple T junction geometry, droplets can now be automatically and precisely controlled on-demand. Specifically, there is ±10% accuracy for droplet generation, ±1.3% monodispersity for 500 μm long droplets and ±4% accuracy for splitting ratios. On-demand merging, mixing, and sorting are also demonstrated as well as the application of a drug screening assay related to neurodegenerative disorders. Overall, this system serves as a foundation for a fully automated system that does not require valves, embedded electrodes, or complex multi-layer fabrication. © 2019 The Royal Society of Chemistry.

Type de document: Article
Mots-clés libres: Automation Drops Neurodegenerative diseases Screening Visual communication Automated control systems Droplet manipulation Embedded electrodes Individual droplets Microfluidic channel Neurodegenerative disorders Pressure-driven flows Sample applications Controlled drug delivery Drug Fluorescent dye Silicone oil Algorithm Article Assay Control system Degenerative disease Drug screening assay Flow Fluorescence Geometry Human Hydrodynamics Image processing Laminar flow Microfluidics Model Pressure driven flow Priority journal Protein aggregation Reaction time Steady state Visual feedback Workflow Devices Lab on a chip Preclinical study Software Drug Evaluation, Preclinical Lab-On-A-Chip Devices
Date de dépôt: 04 juill. 2022 18:03
Dernière modification: 04 juill. 2022 18:03
Version du document déposé: Post-print (version corrigée et acceptée)

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