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SARS-CoV-2 detection by direct rRT-PCR without RNA extraction

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Mérindol, Natacha et Pépin, Geneviève et Marchand, Caroline et Rheault, Marylène et Peterson, Christine et Poirier, André et Houle, Claudia et Germain, Hugo et Danylo, Alexis (2020). SARS-CoV-2 detection by direct rRT-PCR without RNA extraction. Journal of Clinical Virology, 128 . ISSN 1386-6532 DOI 10.1016/j.jcv.2020.104423

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Résumé

Rapid and reliable screening of SARS-CoV-2 is fundamental to assess viral spread and limit the pandemic we are facing. In this study, we compared direct rRT-PCR method (without RNA extraction) using SeeGene AllplexTM 2019-nCoV rRT-PCR with the RealStar® SARS-CoV-2 rRT-PCR kit (Altona Diagnostics). Furthermore, we assessed the impact of swab storage media composition on PCR efficiency. We show that SeeGene and Altona's assays provide similar efficiency. Importantly, we provide evidence that RNA extraction can be successfully bypassed when samples are stored in UTM medium or in molecular water but not when samples are stored in saline solution and in Hanks medium. © 2020 The Author(s)

Type de document: Article
Mots-clés libres: coronavirus COVID19 direct rRTPCR RNA extraction SARS-CoV-2 virus detection
Date de dépôt: 28 sept. 2020 14:20
Dernière modification: 28 sept. 2020 14:20
Version du document déposé: Version officielle de l'éditeur
URI: http://depot-e.uqtr.ca/id/eprint/9255

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